Please organise your manuscript as follows:
The title should be without abbreviations.
This should not exceed 200 words and should describe the core of the paper's message, including essential numerical data. Use four headings: Aims, Methods, Results and Conclusions.
There should be a brief introduction (no heading) followed by these sections: Methods, Results and Discussion.
Tables, figures and images should be inserted into the text where they are first mentioned. State the total number on your submission's title page document.
Separately, attach high-resolution versions of images as .jpg, .tiff, .png or .eps files with you submission.
At the end of the manuscript, briefly list any conflicts of interest.
Use superscript numbers for in-text citations.
Use Vancouver style for references.
Titles of journals are abbreviated according to the style used by Index Medicus for articles in journals. Include all authors where there are four or less; for five or more authors, include only the first three authors followed by "et al." Personal communications and unpublished data should also be cited as such in the text (with the year of the communication).
Check the accuracy and completeness of references at http://www.ncbi.nlm.nih.gov/entrez/query/static/citmatch.html